Chandra’s work has been published, in which he evaluated the ability of pediatric thymus tissue implanted either in leg muscle or under the renal capsule with allogeneic CD34+ hematopoietic stem cells in NSG mice to reconstitute human immune cells and provide a model for rectal HIV-1 transmission. These models were compared with traditional BLT mice or mice that received CD34+ cells only. Renal PedThy mice were similar in immune reconstitution to BLT mice, but Leg PedThy mice in our hands had only transient reconstitution.
Rectal transmission and replication of HIV-1 was the most efficient in BLT mice but lower and more variable in Renal PedThy mice. HIV-1 was poorly transmitted in mice engrafted with CD34+ cells alone or the Leg PedThy mice, which correlated with the frequencies of HIV-1 target cells in the spleen and intestine.
This work was performed in collaboration with fellow MMG colleagues Drs. Sherry Shu and Tom Smithgall as well as Pitt veterinary pathologist, Dr. Lora Rigatti. Chandra also recently presented these data at the International Symposium of Advancing the Humanized Mouse in Boston.
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